Investigations on microbiome of the used clinical device revealed many uncultivable newer bacterial species associated with persistent chronic infections

Introduction. Chronic persistent device-related infections (DRIs) often give culture-negative results in a microbiological investigation. In such cases, investigations on the device metagenome might have diagnostic value. Materials and Methods. The 16SrRNA gene sequence analysis next-generation sequencing (NGS) of clinical were performed to detect bacterial diversity invasive medical devices possibly involved DRIs. Device samples first subjected investigation followed by analysis. Environmental DNA (e-DNA) isolated from was amplification Sanger (n=14). addition, NGS also (n=12). Five only common both methods. Results. Microbial growth observed nine cases; among these, five cases considered significant growth, remaining four either insignificant or contaminated. Culture yielded identical six cases. 16SrDNA microbiome able identify presence rarely described human pathogens, namely Streptococcus infantis, Gemella haemolysans, Meiothermus silvanus, Schlegelella aquatica, Rothia mucilaginosa, Serratia nematodiphila, Enterobacter asburiae, along with some known nosocomial pathogens. Bacterial species as M. silvanus S. nematodiphila that are never reported infection identified. Conclusions. Results small number diverse this pilot study lead path large may validate witnessed. shows culture free, holistic metagenomic approach using could help pathogens chronic